Gammaglobulin factors (Gm and Inv) in New Guinea: anthropological significance

Analysis of the hereditary Gm and Inv gamma-globulin factors of 1669 New Guineans from the Morobe and Eastern Highlands districts and Bougainville Island demonstrates thatthe frequencies of the three Gm alleles present (Gm(a), GM(ab), and Gm(ax)) are similar in general to those in Mongoloids and in particular to those in Southeast Asians and Micronesians. The New Guinea frequencies are distinct from those in other populations, including Australian aborigines. Highly significant differences in frequencies of Gm and Inv alleles occur between Melanesian-and non-Austronesian-speaking New Guineans.     

Searching the world wide Web

The coverage and recency of the major World Wide Web search engines was analyzed, yielding some surprising results. The coverage of any one engine is significantly limited: No single engine indexes more than about one-third of the "indexable Web," the coverage of the six engines investigated varies by an order of magnitude, and combining the results of the six engines yields about 3.5 times as many documents on average as compared with the results from only one engine. Analysis of the overlap between pairs of engines gives an estimated lower bound on the size of the indexable Web of 320 million pages.     

Pathogenic Botryosphaeriaceae associated with <Emphasis Type="Italic">Mangifera indica</Emphasis> in the Kimberley Region of Western Australia

Members of the Botryosphaeriaceae, in particular Lasiodiplodia theobromae, Neofusicoccum parvum, N. mangiferum and Botryosphaeria dothidea, commonly cause stem cankers, dieback and stem end rot of mangoes worldwide. In the current study, eight taxa of Botryosphaeriaceae were identified as canker-associated fungi, pathogens, potential pathogens or endophytes of mangoes in the Kimberley, Australia. These include Neoscytalidium novaehollandiae, Ne. dimidiatum, Pseudofusicoccum adansoniae, P. ardesiacum, P. kimberleyense, Lasiodiplodia sp. 1, L. iraniensis and L. pseudotheobromae. The pathogenicity of a selection of these species toward fruit and branches was tested. All were pathogenic to mango in comparison to the control, with Lasiodiplodia spp. being the most pathogenic. It appears that either geographic isolation or the unique growing conditions in the Kimberley may have provided an effective barrier to the acquisition or establishment of known botryosphaeriaceous pathogens. Wounds caused by mechanical pruning may provide an entry point for infection, whilst severe pruning may increase plant stress.     

Increased tolerance to annual ryegrass toxicity in sheep given a supplement of cobalt

SUMMARY To determine whether oral cobalt supplements could modify the clinical onset of annual ryegrass toxicity, groups (n = 5) of sheep were dosed orally with 0, 4 or 16 mg cobalt/day. After 3 weeks on this treatment, toxic ryegrass seed was added to their feed to provide 0, 0.15 and 0.30 mg corynetoxins/kg body weight, daily. Sheep receiving cobalt ingested 30% more toxin than did unsupplemented sheep before clinical signs developed (P = 0.03). There was no significant difference between groups receiving 4 and 16 mg cobalt. The results showed that cobalt delayed, but did not prevent, the onset of clinical signs of annual ryegrass toxicity.     

The sub-lethal activity of amidines on insects and acarids

A number of straight-chain and cyclic amidines at sub-lethal concentrations elicit a variety of responses from target organisms, usually expressed as a disruption of normal behaviour, leading to reduced feeding of lepidopterous larvae and tick detachment, or reduced fecundity. These effects are particularly evident in Acarina and Lepidoptera, but can also be seen in members of other insect orders. In some previously unpublished experiments, it was demonstrated that some of these compounds act as ‘locomotor stimulants’ to decrease the feeding of lepidopterous larvae. This type of activity probably results from the interaction of these compounds with aminergic in the case of some compounds identified as octopaminergic neurotransmitter/neuromodulator systems in the target organisms. It is not always clear, however, to what extent this type of activity contributes to the control of pests in the field situation.     

Effects of grouping unfamiliar cohorts, high ambient temperature and stocking density on live performance of growing pigs

Ninety-six crossbred intact male pigs (34.5 +/- 3.5 kg BW) were allocated by weight and vocalization score to a 2 x 2 x 2 dynamic experimental design including two stocking densities (1 or 2 m(2)/pig), two temperatures (22 degrees C and 30 degrees C), and two short groupings of unfamiliar cohorts (six pigs as one pig per group, and six pigs per group). The study was conducted over 8 wk, and live weight gain (WTG) and feed intake (FI; as-fed basis) were measured weekly. During the first week, pigs were housed in individual pens from four independent rooms. To group pigs, pen partitions were removed. Pigs were grouped in Rooms 2 and 3 from wk 2 to 4, and in Rooms 1 and 4 during wk 7. Temperature was increased from 22 degrees C to 30 degrees C in Rooms 1 and 2 during wk 4 and 7. Pen partitions were replaced in Rooms 2 and 3 at the end of wk 4 and in Rooms 1 and 4 at the end of wk 7 to return pigs to their individual pens. Grouping pigs decreased FI during wk 3 (15.08 +/- 0.43 vs. 14.03 +/- 0.41 kg P < 0.10), and during wk 7 (17.42 +/- 0.46 vs. 14.24 +/- 0.41 kg; P < 0.01). In addition, grouping had a negative effect (P < 0.001) on WTG at wk 3 (7.38 +/- 0.28 vs. 5.71 +/- 0.28 kg) and at wk 7 (6.70 +/- 0.26 vs. 2.99 +/- 0.26 kg). For grouped pigs, raising the temperature decreased (P < 0.01) WTG (7.49 +/- 0.29 vs. 6.41 +/- 0.29 kg during wk 4, and 3.37 +/- 0.38 vs. 2.62 +/- 0.38 kg during wk 7). Mean FI was decreased (P < 0.01) with the 30 degrees C treatment during wk 7 only (15.49 +/- 0.33 kg at 22 degrees C compared with 12.99 +/- 0.33 kg at 30 degrees C). Compensatory feed intake was evident after the treatments had ceased at wk 6, whereby previously heat-treated grouped pigs had a higher FI (17.97 +/- 0.45 kg) than the animals individually housed at 22 degrees C (12.99 +/- 0.33 kg). Stocking density effects were noted after the grouping and high temperature treatments had ceased. For instance, during wk 5, low-density-housed pigs grew faster (P < 0.001) than their high-density counterparts (9.04 +/- 0.38 vs. 7.49 +/- 0.29 kg). In conclusion, under the conditions of this study, the grouping of unfamiliar cohorts and high ambient temperature treatments had a detrimental effect on pig performance, and these effects were reversible.     

The effect of active immunization against adrenocorticotropic hormone on cortisol, beta-endorphin, vocalization, and growth in pigs

Because the poor growth performance of intensively housed pigs is associated with increased circulating glucocorticoid concentrations, we investigated the effects of glucocorticoid suppression by inducing a humoral immune response to ACTH on physiological and production variables in growing pigs. Grower pigs (28.6 +/- 0.9 kg) were immunized with amino acids 1 through 24 of ACTH conjugated to ovalbumin and suspended in diethylaminoethyl (DEAE) dextran-adjuvant or adjuvant alone (control) on d 1, 28, and 56. The ACTH-specific antibody titers generated suppressed increases in cortisol concentrations on d 63 in response to an acute stressor (P = 0.002; control = 71 +/- 8.2 ng/mL; ACTH-immune = 43 +/- 4.9 ng/mL) without altering basal concentrations. Plasma beta-endorphin concentrations were also increased (P < 0.001) on d 63 (control = 18 +/- 2.1 ng/mL; ACTH-immune = 63 +/- 7.3 ng/mL), presumably because of a release from negative feedback on the expression of proopiomelanocortin in pituitary corticotropes. Immunization against ACTH did not alter ADG (P = 0.120; control = 1,077 +/- 25; ACTH-immune = 1,143 +/- 25 g) or ADFI (P = 0.64; control = 2,719 +/- 42; ACTH-immune = 2,749 +/- 42 g) and did not modify behavior (P = 0.681) assessed by measuring vocalization in response to acute restraint. In summary, suppression of stress-induced cortisol responses through ACTH immunization increased beta-endorphin concentrations, but it did not modify ADG, ADFI, or restraint vocalization score in growing pigs.     

Funisitis associated with leptospiral abortion in an equine placenta

Funisitis, inflammation of the umbilical cord, is well recognized in human placentas. This report describes a case of funisitis associated with leptospiral infection in the placenta of a Thoroughbred foal born prematurely. The umbilical cord had diffuse superficial yellow discoloration along its entire length. Microscopic evaluation showed an exudate of neutrophils admixed with fibrin on the surface. Warthin-Starry staining showed spirochetes in the Wharton's jelly of the umbilical cord. A locally extensive, severe placentitis not involving the star and allantoic cystic hyperplasia were the other lesions observed in the allantochorion. Leptospira funisitis is similar to the funisitis of congenital syphilis in humans, although there are some major microscopic differences. In Leptospira funisitis, lesions were limited to the cord surface, whereas in lesions in human umbilical cords with Treponema pallidum infection, the changes are observed mostly around the vessels and in the Wharton's jelly.